Beneficial influences of germination and subsequent autoclaving of grain legumes on proximate composition, antinutritional factors and apparent digestibility in black tiger shrimp, Penaeus monodon Fabricius

Abstract

This study tested influences of germination and germination in combination with autoclaving on antinutritional factors (ANFs) and digestibility of four grain legumes (field pea, chick pea, black gram and lentil) in black tiger shrimp Penaeus monodon (8 ± 0.5 g). Germination resulted in a significant increase (P < 0.05) in crude protein and decrease in crude lipid content, irrespective of seed variety. Reductions in ANFs were observed to varying extents depending on treatment process, except germination which substantially (P < 0.05) increased trypsin inhibitor levels (TI). However, a significant reduction in TI was observed on subsequent autoclaving. Highest reductions for all the three ANFs were seen in seeds processed by germination in combination with autoclaving. Higher apparent digestibility co-efficients (ADCs) for dry matter (73.4–84.5%) and crude protein (89.1–99.6%) were obtained in seeds processed by germination in combination with autoclaving, and the trend was similar for all tested seeds. ADCs for crude fibre and nitrogen-free extract were ranged between 32.43–46.46 and 75.49–82.13, respectively. To conclude, irrespective of type, seeds processed by germination in combination with autoclaving were low in ANF and showed improved proximate composition as influenced by germination. Both of these positive influences were reflected in higher ADCs.