Abstract
ObjectiveAtopic dermatitis (AD) is a chronic inflammatory skin disease mainly caused by immune stimuli. The current study was conducted to investigate the effects of ROCEN and to compare it with betamethasone (Beta) on mice subjected to AD.MethodsFirst, the safety of topical ROCEN was tested to determine possible sensitization induction in vivo. Then, the mice were subjected to oxazolone (Oxa) to induce chronic AD. Consequently, they underwent treatment with ROCEN and Beta. Scratching and wiping behaviors related to dermatitis were evaluated in treated animals for 35 days. The histopathology and immunohistochemistry (IHC) analysis of interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) cytokines were performed on the dorsal skin of the treated mice.ResultsTopical administration of ROCEN and Beta to the dorsum of sensitized mice for 5 weeks significantly alleviated scratching and wiping symptoms and reduced erythema, scaling, and edema in the skin of the mice with AD. Moreover, histological indices showed that ROCEN effectively reduced leucocyte infiltration and improved skin healing parameters in treated AD mice. Application of ROCEN or Beta reduced IHC markers including IL-8 and TNF-α significantly.ConclusionROCEN alleviated the AD symptoms similar to betamethasone in an experimental animal model.
Highlights
Atopic dermatitis (AD) is a highly prevalent, chronic, inflammatory skin disease [1] characterized by severe itching, a mild to severe rash, edema, hemorrhage, and erosion of the skin surface [2]
Skin severity reduction by ROCEN After repeated use of Oxa, skin dryness occurred with mild erythema and edema in AD mice followed by thick scars
Our findings suggest a hypothesis that topical ROCEN can potentially reduce pro-inflammatory cytokines such as tumor necrosis factorα (TNF-α) and IL-8, resulting in lipid homeostasis of the skin barrier in the lesion site and improvement of dermatitis symptoms
Summary
Atopic dermatitis (AD) is a highly prevalent, chronic, inflammatory skin disease [1] characterized by severe itching, a mild to severe rash, edema, hemorrhage, and erosion of the skin surface [2]. It is associated with a variety of immunological mechanisms and environmental and even neuropsychological factors may have a role in the development of this disease [3,4,5,6]. Goudarzi et al BMC Complementary Medicine and Therapies (2021) 21:226 inflammatory cytokines such as TNF-α decrease the level of long-chain free fatty acids and ceramides, which affects the lipid organization distribution and results in skin injury [10]. New formulations and drugs are always of interest to improve the quality of life of AD patients
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