Abstract
Objective: The primary aim of the study was to find out a suitable effective dose of metanil yellow that can cause alterations of lipid peroxidation and catalase activity in the heart in vitro and to determine the effectiveness of graded doses of Coriandrum sativum as an antioxidant in preventing lipid peroxidative damage to cardiac tissue and back up catalase activity. Methods: Metanil yellow at different doses (10, 25, 35, 50 µl in phosphate buffer) were applied on isolated hircine heart homogenate (10%) in in vitro followed by estimation of lipid peroxidation and catalase activity after completion of the incubation period. Another set of the experiment included incubation of the heart homogenates with graded dilutions of ethanolic leaf extracts of Coriandrum sativum (CsEth) at concentrations of 10, 20, 30, 40 µl in phosphate buffer medium followed by quantification of the same biochemical parameters as above. This was followed by co-administration of the selected effective dose of metanil yellow (35 µl) and ethanolic leaf extract of Coriandrum sativum (30 µl) on heart homogenate to track the variation in lipid peroxidation and catalase activity in heart homogenate in vitro.Results: Metanil yellow significantly lowered catalase activity in isolated cardiac tissue that was reflected in elevated lipid peroxidation assuming free radical induced oxidative injury to cardiac tissue (p<0.01). However ethanolic leaf extract of Cordiandrum sativum buffered such changes (p<0.01) significantly, presuming a protective action against oxidative cardiac damage.Conclusion: It may be concluded that ethanolic leaf extract of Coriandrum sativum is a promising cardioprotective remedy not only in mitigating free radical mediated lipid peroxidative damage to cardiac tissue but also in reinstating normal catalase activity that may be used an unique herbal based remedy for such cardiac ailments in future.
Highlights
Reactive oxygen species (ROS) are an important group of molecules that are generated usually by oxygen slippage either during mitochondrial electron transport or extra-mitochondrial electron transport systems [1]
We found that except saponin all three other constituents were present in the ethanolic extract of Coriandrum sativum
We found that co-treatment of heart tissues of goat in vitro ethanolic extract of Coriandrum sativum (EthCs)at various doses (10 μl, 20 μl, 30 μl, 40 μl,50 μl) protected the level of lipid peroxidation in goat heart from being increased in a dose response manner compared to that of metanil yellow treated heart
Summary
Reactive oxygen species (ROS) are an important group of molecules that are generated usually by oxygen slippage either during mitochondrial electron transport or extra-mitochondrial electron transport systems [1]. An imbalance between the generation of such free radicals and scavenging activity of these free radical by cellular antioxidant enzyme cascade may be a cause for generation of oxidative stress. Oxidative stress may be the etiology of a multitude of ailments ranging from neurological, haematological, immunological, cardiovascular, and genetic to developmental disorders [2]. Mammalian heart is highly aerobic and oxygen demanding organ. Cardiac tissue is normally dynamic with highly metabolic demand and mitochondrial rich myocardial work out may be a source of oxygen slippage turning out as oxidative injury to the heart. ROS generation in the heart may affect normal myocardial contractility induce maladaptive myocardial remodelling and subsequently, heart failure may be an outcome [3]
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