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Abstract
In the bioethanol production process, high solid saccharification and glucose/xylose co-fermentation are important technologies for obtaining increased ethanol concentrations; however, bench-scale studies using combinations of these methods are limited. In this study, we hydrolyzed high solid concentration of milled eucalyptus using commercial enzymes and obtained 138.4 g/L total monomeric sugar concentration. These sugars were fermented to 53.5 g/L of ethanol by a xylose-utilizing recombinant Saccharomyces cerevisiae strain, MA-R4. These experiments were performed in bench scale (using 50 L scale solid mixer and 70 L scale fermenter). The results obtained in this study were comparable to our previous results in laboratory scale, indicating that we successfully achieved an efficient high solid saccharification and glucose/xylose co-fermentation system in bench scale.
Highlights
In the bioethanol production process, high solid saccharification and glucose/xylose co-fermentation are important technologies for obtaining increased ethanol concentrations; bench-scale studies using combinations of these methods are limited
The results obtained in this study were comparable to our previous results in laboratory scale, indicating that we successfully achieved an efficient high solid saccharification and glucose/xylose co-fermentation system in bench scale
We have previously studied the pretreatment methods that do not require the use of chemicals, such as milling treatment [3, 4], namely, lignocellulosic material is ground into \1 mm by a cutter mill prior to hydrothermal treatment
Summary
In the bioethanol production process, high solid saccharification and glucose/xylose co-fermentation are important technologies for obtaining increased ethanol concentrations; bench-scale studies using combinations of these methods are limited. We hydrolyzed high solid concentration of milled eucalyptus using commercial enzymes and obtained 138.4 g/L total monomeric sugar concentration These sugars were fermented to 53.5 g/L of ethanol by a xylose-utilizing recombinant Saccharomyces cerevisiae strain, MA-R4. We have previously studied the pretreatment methods that do not require the use of chemicals, such as milling treatment [3, 4], namely, lignocellulosic material is ground into \1 mm by a cutter mill prior to hydrothermal treatment This process enables the partial hydrolysis of hemicellulose and weakens bonds among cellulose, hemicellulose, and lignin. Monomeric sugar conversions of [80 g/L were produced by the saccharification process, with [35 g/L of ethanol obtained
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