Belowground facilitation of plant mixtures on rhizosphere soil of Commelina communis grown on extremely Cu- and Cd-contaminated mine: From soil quality to bacterial community

Abstract

Plant–plant interactions exert positive effects under stress conditions, but their impact on heavy metal-contaminated rhizosphere soil quality and bacterial community remains unclear. In this work, the rhizospheric soil of Cu-accumulator Commelina communis and neighboring plants grown in a heavily contaminated copper mine was analyzed to elucidate such belowground facilitation. Physicochemical properties and microbial characteristics of rhizospheric soil of control C. communis (far from neighboring plants), facilitated C. communis (near neighboring plants), and neighboring plants were compared. The average values of total organic carbon, NH4+-N, NO2−-N, NO3−-N, available phosphorus, urease, and phosphatase in the C. communis rhizosphere increased by 0.82-, 0.64-, 0.44-, 0.06-, 0.10-, 0.36-, and 0.30-fold in the presence of surrounding plants, respectively. The relative abundance of oligotrophic bacteria (Chloroflexi) decreased, whereas copiotrophic bacteria (Proteobacteria) increased in plant species mixtures. Venn graph and partial least squares discriminant analysis indicated that the composition of the bacterial community in the facilitated C. communis rhizosphere showed higher similarity to neighboring plants than to control C. communis. Among 49 predicted functional groups analyzed by FAPROTAX analysis, chemoheterotrophy (36.04 %) involved in carbon cycles had the highest relative abundance and increased in the facilitated C. communis rhizosphere due to the positive response of 193 OTUs (mainly belonging to Proteobacteria and Actinobacteriota) under plant–plant interactions. The increased relative abundance of Methylocella and Bradyrhizobium resulted in improved nitrogen fixation in the C. communis rhizosphere, whereas nitrate reduction with its main performer Rubrobacter was inhibited. The ecological function of rhizobiomes of the facilitated C. communis and neighbors also showed higher similarity compared with that of the control C. communis. These results show the role of neighboring plants in modulating soil quality and rhizobiomes of plant remediators under stress and more indoor garden experiments need to be conducted to verify such belowground facilitation.