Beijing as the hub of CRF07_BC transmission from the intravenous drug users to men who have sex with men in China.

Abstract

The HIV-1 CRF07_BC strain has been increasing in frequency in China, is now the major recombination subtype in the men who have sex with men (MSM) population. However, to date, the evolutionary history of CRF07_BC in MSM in China has not been explored in greater detail. All available partial gag, pol and env segments of CRF07_BC were obtained from the Los Alamos HIV database. We performed phylogenetic analysis for these fragments by using the Bayesian Markov Chain Monte Carlo method, as implemented in BEAST. We estimated the history of viral migration between different areas over time using the discrete Bayesian phylogeographical method. Spatial reconstruction showed that Yunnan had the highest root state posterior probability (0.89 in pol, 0.60 in gag, 0.87 in env). The virus migrated from Yunnan to Beijing in 1990s through intravenous drug users (IDU). The highest root state posterior probability of MSM cluster is Beijing (0.93 in pol, 0.90 in gag and 0.37 in env). In the beginning of 2000s, Beijing became the primary epicenter as CRF07_BC had expanded their geographical distribution to many provinces in China in MSM population. Guangdong and Shanghai were secondary epicenters, with a large extent of viruses. This study highlighted the spatial and temporal spread of CRF07_BC in MSM population in China. It revealed that Beijing was the hub of CRF07_BC transmission from IDU to MSM in China.