Behavioural, Teratogenic and Genotoxic Effects of Antibacterial Compounds, Triclocarban and Triclosan, in Hydra vulgaris.

Abstract

Triclocarban (TCC) and triclosan (TCS) are antibacterial compounds used in household, veterinary, industrial and personal care products, which are known to be environmental pollutants and also toxic to organisms. The toxicological effects of these antibacterial chemicals on higher organisms have been studied in detail. But in lower invertebrates like hydra, it is still rare and yet to be explored. In this study, the toxicological effects of these two antibacterial compounds in Hydra vulgaris was performed to clearly understand the organismal, developmental, molecular and behavioural changes. Both TCC and TCS are toxic with respective LC50 values of 0.09 and 0.25 mg/L, whereas TCC is comparatively more toxic than TCS. The structural damage of battery cell complexes (BCCs) on the tentacles was observed and ultimately made prey capturing difficult. It was evident that TCC and TCS exposure caused developmental toxicity by affecting reproduction and regeneration in H. vulgaris at higher sublethal doses (0.045 and 0.125 mg/L, respectively). TCC and TCS also caused DNA damage resulting in apoptosis. This study further reveals that these two antibacterial compounds are teratogenic and genotoxic in the organisms.