Behavioural correlates of conditioned ventilatory responses to hypoxia in rats

Abstract

To examine the possible contribution of behavioural arousal to ventilatory conditioning, we performed a differential conditioning experiment using two odours as the paired conditioned stimulus (CS+) and unpaired conditioned stimulus (CS−) and a hypoxic mixture (7.5% O 2) as the unconditioned stimulus (US) in 24 adult male rats. Vanillin was the CS+ and rose the CS− in half the rats, and vice versa in the other half. Each rat underwent 26 paired CS+/hypoxia trials and 26 CS− trials in alternation, followed by two CS+ only and two CS− trials to test for conditioning. Analysis of breathing variables and behavioural scores during the test showed two qualitatively different conditioned responses. The initial conditioned response was characterised by short breath durations (TT), frequent sniffing episodes, and arousal responses. Following this, a specific, conditioned increase in tidal volume (VT) and levelling off of sniffing and motor activities occurred. The early TT-response and late VT-response to CS+ both contributed to an increase in ventilation (VI). The present data show that the association of an odour and hypoxia elicits a biphasic ventilatory conditioned response, of which the first component is integrated into conditioned arousal.