Abstract
A radioactive complex AP 2.3 (A: bovine serum albumin, P: radioactive palmitic acid) has been prepared and incubated at pH 9 and 65°C for 60 min. Analysis by disc gel electrophoresis revealed three zones: zone 1, undenatured monomer; zone 1′, modified monomer; and zone 2, dimer. Counting of sliced gels indicated that only zone 1 was radioactive, meaning that fatty acids are released in the process 1→1′, but not in the process 1′+1′→2. In other words, fatty acids are released from albumin when native albumin is unfolded to form component 1′ during the incubation. The fatty acids released are concentrated on a particular species of albumin molecule which is not changed to component 1′. The percentage of component 1 ( p) was 37%. This value agrees with that calculated by the equation which was proposed by us in a previous paper, p = (v/6) × 100%. In the present case v is 2.3.
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