Abstract
Osteoclasts isolated from 3-4 days rats were cultured on laser irradiated bovineroot dentin to determine the behaviour of the osteoclasts, especially the cell adhesion as well as the resorption of dentin. Methods were as follows.. The cells were stained by FITC-marked phalloidin and observed under fluorescence microscopy.2. The cells were stained with TRAP and observed under microscopy.3. The cells were observed under SEM.Following results were obtained.Osteoclasts and resorption pits were observed on the unirradiated dentin, however, they were not seen on the laser irradiated dentin. Laser exposure to dentin seemed to inhibit the adhesion of osteoclasts.
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