Behaviour of Nitinol in osteoblast-like ROS-17 cell cultures

Abstract

Nickel titanium shape memory metal alloy Nitinol (NiTi) has been used in dental wares and in gastrointestinal surgery. Nitinol is a promising implant material in orthopedics, but its biocompatibility, especially in long-term implantation is not confirmed yet. We studied Nitinol's effect on a cell culture model. Comparisons to stainless steel, pure titanium and pure nickel were performed. The effects of Nitinol on cell death rate, the apoptosis rate and the formation of focal contacts were studied on rat osteosarcoma cell line ROS-17 in 48-h cultures. The cell death rate was assessed with combined calcein–ethidium–homodimer labelling. The amount of dead cells/1000 cells were as follows: four in the NiTi, 21 in the Stst, 4.8 in the Ti and 51 in the Ni group. In the NiTi and Ti groups, the number of dead cells was significantly lower ( p⩽0.01) than in Ni group. The rate of apoptosis was detected with TUNEL-assay. The assay results were: 1.93 apoptotic cells/1000 cells in the NiTi, 1.1 in the Stst, 2.98 in the Ti and 0.62 in the Ni group. A comparison of these two results shows that 48% of the dead cells were apoptotic in the NiTi, 5.6% in the Stst, 62% in the Ti and only 1.8% in the Ni group. The focal contacts were stained with a paxillin antibody and counted. There were marked differences in the number of focal contacts per unit area compared to NiTi (774 focal contacts): 335 in Stst ( p⩽0.01), 462 in Ti ( p⩽0.01) and 261 in Ni ( p⩽0.005). Our results show that NiTi is well tolerated by the osteoblastic type ROS-17 cells.