Abstract
Four maize lines, two inbreds from northern Portugal (AMARELO and PIGARRO) and two hybrids (HB3, obtained from the cross of inbred lines adapted to soils rich in Al, and ACCO1148) that present different Al-tolerances when cultivated in field conditions, were screened for physiological and biochemical behavior in three experimental growth systems — hydropony, washed quartz sand and early germination on filter paper — in the presence of different Al concentrations. Plants in hydropony were analysed for growth parameters, nitrate uptake, pH-change of the nutrient solution and Al accumulation in shoots and roots. In vivo nitrate reductase activity (NRA) and nitrate content of the leaves were measured in plants developed in quartz sand. Root tips of three day old seedlings of ACCO1148 germinated on moistened paper were submitted to in vivo P-NMR spectroscopy to evaluate the intracellular pH and to identify mobile phosphorus compounds.
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