Behaviour and reactivity of growing rabbits housed in collective pens: Effects of floor type and stocking density at different ages

Abstract

At 33 d of age, 376 crossbred rabbits of both genders were housed in sex-mixed groups in 16 open-top collective pens (1.68 m<sup>2</sup>) in a factorial arrangement, with 2 types of flooring (wooden slats <em>vs.</em> plastic grid, W <em>vs.</em> P) and 2 stocking densities (12 <em>vs.</em> 16 animals/m<sup>2</sup>). Behavioural recordings (time budget), reactivity towards unknown humans (tonic immobility and human approach tests) and environment (open-field and novel object tests) and hair and faeces sampling for corticosterone analysis were performed during the 8<sup>th</sup> wk of age and at a pre-slaughter age (during the 11-12<sup>th</sup> wk of age). The effects of age and gender were also taken into account. Rabbits housed in W pens rested more in the crouched position (41.4 <em>vs.</em> 35.5% of the observed time) (<em>P</em><0.001) and showed lower allogrooming (<em>P</em>=0.05), running (<em>P</em><0.01) and biting pen elements (<em>P</em>=0.01) than those housed in P pens. The percentage of rabbits that interacted with humans during the human approach test (24.0 <em>vs.</em> 48.1% of rabbits in the pen; <em>P</em><0.01) and number of rabbit-object contacts during the novel object test (on av. 50.4 <em>vs.</em> 87.2; <em>P</em><0.001) were lower for rabbits in W pens compared to those in P pens. During the open-field test, the percentage of rabbits that spontaneously entered the arena was lower for rabbits from W pens than for those from P pens (60.0 <em>vs.</em> 72.5%; <em>P</em><0.05). Finally, the hair corticosterone level was higher in the former than in the latter rabbits (on av. 14.0 <em>vs.</em> 12.5 ng/g; <em>P</em><0.05). As the stocking density increased, only the time spent resting increased (66.7 to 69.1% observed time; <em>P</em><0.01), and the percentage of rabbits that spontaneously entered the arena during the open-field test decreased (73.8 to 58.8%; <em>P</em><0.01). When age increased, the rabbits were less active at the reactivity tests and interacted less with an unknown object or person. Differences according to gender were weak. In conclusion, the wooden slatted floor challenged the welfare of growing rabbits as it constrained their movement, conditioned their reactivity towards a new environment, and increased their stress level, whereas the increase in stocking density did not impair rabbit welfare.