BEHAVIORAL STUDIES OF WISTAR RATS IN ROTENONE INDUCED MODEL OF PARKINSON’S DISEASE

Srimathi Priyanga K,Vijayalakshmi K,Selvaraj R

doi:10.22159/ijpps.2017v9i11.21465

Srimathi Priyanga K, Vijayalakshmi K + Show 1 more

Open Access

https://doi.org/10.22159/ijpps.2017v9i11.21465

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Abstract

Objective: The objective of the study was to determine the behavioral activities of Wistar rats induced with rotenone. Methods: Thirty-six male Wistar rats were taken for the study and divided into six groups of six rats each. Group-I is the vehicle-treated, Group–II animals were induced with rotenone (3 mg/kg/bwt) by i. p. Group-III were co-treated with rotenone and L-DOPA (10 mg/kg/bwt) orally, Group-IV were co-treated with rotenone and quercetin (25 mg/kg/bwt) orally, Group-V were co-treated with rotenone and hesperidin (50 mg/kg/bwt) orally, Group-VI were treated with rotenone, quercetin and hesperidin in the same dosage regime for 60 d. The behavioural tests, such as open field test, ladder climbing test and hanging wire test were performed. The biochemical parameters such as urea, creatinine and activities of ALT and AST were also analysed.Results: All data are expressed as the mean±SD. Disability was noted in the behaviour of rats induced with Parkinson’s disease (PD). The deficits in behavioral activity were significantly changed when compared with an induced group (p<0.001) and biochemical parameters due to rotenone were significantly (p<0.001) restored by co-treatment with quercetin and hesperidin.Conclusion: In our in vivo study, we have demonstrated the combination of quercetin and hesperidin to serve as neuroprotective compounds by improving the behavioral abnormalities and restoring the biochemical parameters. Hence, these powerful antioxidants may protect brain cells.

Highlights

Parkinson’s disease (PD) is one of the most common chronic neurodegenerative disorder
We investigated the neuroprotective effect of quecetin and hesperidin against rotenone-induced animal model for Parkinson’s disease (PD) by analysing biochemical changes along with a behavioral alteration in a rat model
The current results indicated that LDOPA+rotenone, quercetin+rotenone and hesperidin+rotenone group was significantly increased when compared with rotenone group

Summary

Introduction

Parkinson’s disease (PD) is one of the most common chronic neurodegenerative disorder. It is characterized by a variety of motor impairment like bradykinesia, rigidity, tremor and postural instability. Parkinson’s disease(PD) is characterized by the severe loss of dopaminergic (DAergic) neurons in the substatina nigra pars compacta (SNpc) and the presence of Lewy bodies (LBs),which are present in neurons of the central nervous system [1, 3]. Mitochondria is involved in electron transport chain and rotenone is interfering in this pathway and it leads to mitochondrial damage. It inhibits the electron transfer from the center of iron-sulfur in complex I to ubiquinone. Rotenone interferes with the ATP synthesis [4]

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