Behavioral and olfactory responses of grasshopper hatchlings, Melanoplus sanguinipes, to plant odours and volatile compounds

Abstract

Behavior and olfactory responses of grasshopper hatchlings,Melanoplus sanguinipes (F.), to odours from plant foliage and volatile compounds were tested using a glass Y-tube olfactometer and electroantennogram (EAG) techniques respectively. In single choice trials, newly hatched hoppers were much more sensitive to the odour from intact leaves and chopped foliage of ryegrass and wheat than other plants. Chopped sorghum leaves, but not stem-cut sorghum, were also significantly attractive. The orientation responses of grasshopper hatchlings to these plants were highly consistent with those of last instar hoppers and adults. When ryegrass was employed as the control, the odour from stem-cut alfalfa was more attractive. There was no significant difference in hopper orientation responses to the odours from chopped seedlings of sorghum, alfalfa, wheat or ryegrass. However, significantly more hoppers preferred the chopped ryegrass control to chopped Louisanna sage. Measurement of the EAG response of first instar hoppers to these plant odours showed that the odour of Louisanna sage elicited the greatest response amplitudes. In olfactory tests using different volatile components, Z-3-hexenol, E-3-hexenol, Z-hex-3-enyl acetate, E-2-hexenal and hexenal gave greater EAG responses than geraniol and 1-octen-3-ol. These results are also consistent with comparable data from adults. Newly hatched grasshoppers had similar EAG response profiles to plant materials and chemicals to those of adults, although the absolute EAG values of young hoppers were much lower than those of adults. Therefore, newly hatched hoppers were able to distinguish plants from an air control, and even host plants from non-host plants, and the feeding experience of hoppers probably has little influence on their subsequent ability as adults to identify and locate food plants.