Abstract
IntroductionNeuropathic pain is defined as pain arising as a direct consequence of a lesion or disease affecting the somatosensory system and is common after surgery. Neuropathic pain can persist without an obvious injury. In this study we aim to validate a murine chronic constriction injury model as a model for neuropathic pain research and determine if silk or catgut ligatures induced most stable neuropathic pain behavior.MethodsIn this study mice underwent chronic constriction or sham surgery. Mice were tested on cutaneous hyperalgesia with the cumulative reaction time in the acetone test, on allodynia with the cumulative reaction time and number of lifts in the cold plate test and the maximal force before withdrawal in von Frey test.ResultsIn the acetone test neuropathic pain was seen in CCI mice, but not in sham mice. Hyperalgesia was present postoperatively in CCI mice compared with preoperatively. In the cold plate test cumulative reaction time and number of lifts were higher in the ipsilateral hind paw than in the contralateral hind paw and sham mice. Postoperative measurements were higher than preoperatively. In the von Frey test the postoperative measurements were lower in the ipsilateral hind paw than preoperatively, while the contralateral hind paw showed an increase in maximal force before withdrawal. The contralateral hind paw showed more difference with sham mice than the ipsilateral hind paw. Silk ligatures showed more stable neuropathic pain behavior. In the acetone test, the cold plate test and the von Frey test the mice scored higher on neuropathic pain having silk ligatures, compared with catgut ligatures.ConclusionIn this study we validated a murine CCI model for neuropathic pain behavior. In the murine CCI model it appears that silk ligatures demonstrate more stable neuropathic pain behaviors than catgut ligatures in de CCI model.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-015-1009-4) contains supplementary material, which is available to authorized users.
Highlights
Neuropathic pain is defined as pain arising as a direct consequence of a lesion or disease affecting the somatosensory system and is common after surgery
In this study we aim to validate a model of neuropathic pain in mice and investigate whether silk or catgut ligature material is more effective in inducing neuropathic pain
In this study we demonstrated that a chronic constrictive injury (CCI) model in mice can induce neuropathic pain behaviors comparable to neuropathic pain signs and symptoms in humans
Summary
Neuropathic pain is defined as pain arising as a direct consequence of a lesion or disease affecting the somatosensory system and is common after surgery. Neuropathic pain is defined as pain arising as a consequence of a lesion or disease affecting the somatosensory system and is common after surgery (Treede et al 2008). Neuropathic pain is associated with poor physical and mental health and adversely affects quality of life (Freynhagen & Bennett 2009; Leung & CM Cahill 2010). Considering the above neuropathic pain adds to the burden of direct and indirect medical cost for our society (Leung & C.M. Cahill 2010), as there are direct medical costs, loss of the ability to work, loss of caregivers' ability van der Wal et al SpringerPlus (2015) 4:225 to work and possibly greater need for institutionalization or other living assistance (O'Connor 2009)
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