Abstract
The number and the form of mitochondria in vegetatively growing yeast, Saccharo-myces cerevisiae, have previously been determined by the use of serial thin sections of entire cells. It was found that the number of mitochondria is directly related to the growth phase: logarithmic phase cells contain few mitochondria, less than 10, while stationary phase cells have numerous organelles, from 30 to 50 individual mitochondria. A single mitochondrion was found in 2 log phase, glucose-repressed cells, but all other cells had more than one mitochondrion. In the present study, the behavior of the chondriome during meiosis and sporulation in yeast was followed by the serial section technique and was compared to that in vegetative cells.Diploid strains Z193, Z239-6B6B and s4l were obtained from R. and M. Esposito and S. Klapholz (Univ. of Chicago) and serial sections of strain 112 were kindly provided by D. Zickler (Univ. Paris-Sud). Cells were fixed in glutaraldehyde, treated with glusulase (Endo Lab.) or zymolyase (Kirin Brew. Co.) to remove the cell wall, and post-fixed in Dalton's chrome osmium.
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