Abstract
Abstract Downy mildew (Plasmopara viticola) is the main grapevine disease in humid regions. In the present investigation, marker-assisted selection (MAS) was used to develop grapevine lines homozygous in loci Rpv1 and Rpv3 for resistance against P. viticola. The experimental populations UFSC-2013-1 (n = 420) and UFSC-2013-2 (n = 237) were obtained by self-pollination of two 𝐅 𝟏 full-sib plants, originated from a cross between two distinct breeding lines containing the downy mildew resistance loci Rpv1 and Rpv3 in heterozygosity. The two experimental populations were genotyped with four microsatellite markers flanking the two downy mildew resistance loci. Among 637 genotyped plants, 300 (48.2%) were homozygous for at least one resistance locus and 10 (1.57%) were homozygous for both Rpv1 and Rpv3 alleles. These 10 plants challenged with P. viticola inoculum showed a clearly enhanced level of resistance. These plants have a great potential as resistance donors in grapevine breeding.
Highlights
The diploid obligate biotrophic oomycete Plasmopara viticola (Berk & Kurt) causes downy mildew, one of the main diseases that reduce grape (Vitis vinifera) production (Caffi et al 2010). This yield loss is not confined to one season; early leaf death and defoliation caused by the disease weakens the vine and leads to poor harvests in subsequent seasons (Matasci et al 2008)
The pathogen was found originally in North America on wild Vitis (Olmo 1986); it has since spread across the globe to many countries, including Brazil, by introduction of American vines contaminated with the pathogen (Santos-Neto 1955)
Genotyping with four microsatellite markers linked to resistance alleles Rpv1 and Rpv3 classified plants according to the number of resistance alleles present in each plant
Summary
The diploid obligate biotrophic oomycete Plasmopara viticola (Berk & Kurt) causes downy mildew, one of the main diseases that reduce grape (Vitis vinifera) production (Caffi et al 2010). This yield loss is not confined to one season; early leaf death and defoliation caused by the disease weakens the vine and leads to poor harvests in subsequent seasons (Matasci et al 2008). For more than two centuries, V. vinifera and Muscadinia rotundifolia, a donor of resistance genes, have been bred with the objective of combining high-quality fruit production with effective disease resistance (Olmo 1986, Alleweldt and Possingham 1988).
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