Abstract
The hemolytic behavior of rabbit and human erythrocytes in water‐glycerin and water‐propylene glycol solutions was investigated. Complete hemolysis of erythrocytes took place in all propylene glycol solutions and most glycerin solutions. Aqueous solutions containing 50, 60, and 70 per cent glycerin prevented complete hemolysis of rabbit erythrocytes but not human erythrocytes. The addition of sodium chloride to various glycerin solutions prevented hemolysis. The addition of sodium chloride to propylene glycol solutions prevented hemolysis of rabbit erythrocytes in 5–30 per cent solutions and of human erythrocytes in 5–40 per cent solutions. When possible, the data were used to calculate van't Hoff i values for sodium chloride in various water‐glycerin and water‐propylene glycol solutions. Unusual behavior was displayed by erythrocytes in 40–50 per cent propylene glycol solutions. The addition of sodium chloride to solutions containing 50 per cent or more of propylene glycol did not prevent complete laking of red blood cells.
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