Abstract
We used bioluminescent Ralstonia solanacearum YN5 obtained by transforming R. solanacearum OE1-1 with pNP126 carrying the luxCDABE operon of Vibrio fisheri and a promoter region derived from genomic DNA of Burkholderia glumae to observe the behavior of bacteria in tomato plants and the development of bacterial wilt in tomato. Our study demonstrated that the degree of bacterial multiplication in the root and collar after bacterial invasion and the degree of bacterial spread to the upper stem were important factors that determined the susceptibility and the resistance of tomato to bacterial wilt.
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