Abstract
In tissue engineering, surface modification has becomes one of the leading methods to enhance initial cell attachment and subsequent cellular growth, differentiation and tissue formation. This work studied growth and behavior of primary bovine articular chondrocytes on self-assembled multilayer nanofilms composed of: polyelectrolytes [poly(styrene sulfonate) (PSS), poly-L-lysine (PLL), poly-D-lysine (PDL), chondroitin sulfate (CS), poly(ethyleneimine) (PEI), poly(dimethyldiallylammonium chloride) (PDDA), poly(ethylene glycol) amine (PEG - NH2)] and proteins [bovine serum albumin (BSA), collagen, fibronectin, laminin]. These biomaterials were used to build mono-, bi-, and tri-layer nanofilm architectures. Potential cytotoxic effects were assessed using Live/Dead assay and cell proliferation was quantified using MTT assay. Bright field and fluorescence microscopy were used to analyze chondrocyte morphology. ImageJ software was used to analyze the number, mean area, circularity and Feret's diameter of viable cells. Cumulative results demonstrated that chondrocyte growth; proliferation and functionality were dependent on initial cell density, nanofilm thickness and material composition of nanofilms.
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