Abstract
The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.
Highlights
Common carp (Cyprinus carpio L.) is one of the important commodities in Indonesia freshwater aquaculture
Our results showed that behavior, histopathology, Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea and creatinine levels of rat fed with control, NT- and TG-common carp meal (Ccm) diets were similar
Body Weight Gain According to rat weight of each treatment, it found that rats fed on Ccm supplemented diet had higher body weight (p
Summary
Common carp (Cyprinus carpio L.) is one of the important commodities in Indonesia freshwater aquaculture. As a part of common carp genetic quality improvement program in Indonesia, transgenic common carp fish whose second generation expressed Nile tilapia growth hormone (GH) gene has been produced (Alimuddin et al 2012). These fishes exhibit molecular marker associated with KHV infection resistance. The result of limited field test showed that growth of GH transgenic common carp has 27.5-338.8% higher compared to non-transgenic fish (Alimuddin et al 2013).
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