Abstract
An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after setting on the stem explants, the number and the size of shoots, the root size, the presence or absence of callus and the survival, were evaluated. The survival was monthly recorded during 8 months. Taking into account the explant development, four media were proposed for collection preservation. One of them, Ga, with a reduction of salts, sucrose, 6-benzoaminopurine, omitting adenine sulphate and 1-naphthalene acetic acid, is the most economical. Behavior observations of the 32 clones in the 15 medium compositions showed the influence of the genotype of the clones.
Highlights
Demand for germplasm preservation of various plant species has recently increased due to pollution, climate change, natural destruction and human impact on the ecosystem biodiversity (Son et al, 1997)
In vitro culture is advantageous for different reasons a) entire sets of genetic materials can be copied through regeneration schemes, b) rapid proliferation is achievable, c) disease-free plants can be produced and maintained, minimal space is required, d) risks related to environmental changes are avoided and f) behavior studies of clones in different medium compositions
We studied 15 different chemical compositions of in vitro culture media that allowed the conservation of healthy plants with vigorous stems, green leaves, developed roots and slow growth avoiding callus formation, during a period of time superior of three months without subculture
Summary
Demand for germplasm preservation of various plant species has recently increased due to pollution, climate change, natural destruction and human impact on the ecosystem biodiversity (Son et al, 1997). The P. tremula collection was subcultured in the Aspen Culture proliferation Medium (ACM) described by Ahuja (1983). In vitro culture is advantageous for different reasons a) entire sets of genetic materials can be copied through regeneration schemes, b) rapid proliferation is achievable, c) disease-free plants can be produced and maintained, minimal space is required, d) risks related to environmental changes are avoided and f) behavior studies of clones in different medium compositions. This characterization gave useful information about the selected clones. These conditions may contribute to reduce the cost and the number of manipulations
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