Beagle dog 90-day oral toxicity study of a novel coccidiostat \u2013 ethanamizuril

Keyu Zhang,Feiqun Xue,Yingchun Liu,Shuya Wei,Haihong Zheng,Chunmei Wang,Chenzhong Fei,Lifang Zhang,Xiaoyang Wang,Shusheng Tang

doi:10.1186/s12917-020-02655-2

Abstract

BackgroundTriazine coccidiostats are widely used in chickens and turkeys for coccidiosis control. Ethanamizuril is a novel triazine compound that exhibits anticoccidial activity in poultry. This study was designed to evaluate the subchronic toxicity of ethanamizuril in beagle dogs at doses of 12, 60 or 300 mg/kg/day in diet for 90 days.ResultsEthanamizuril was well tolerated at low and middle dosages in beagle dogs, and no drug-related toxical effects were observaed in terms of survival, clinical observations, organs weight and damage in these dose groups. However, in high dose administration group, food consumption and histologic changes in kidneys were noticed in both sexes of beagle dog, although the renal lesions were finally resolved at the end of 4 weeks exposure of ethanamizuril.ConclusionsNo-observed-adverse-effect level (NOAEL) was considered for ethanamizuril at dose of 60 mg/kg/day in Beagle dog. This result added toxicity effects of ethanamizuril to the safety database, which might guide safely using of ethanamizuril as a novel coccidiostat.

Highlights

Triazine coccidiostats are widely used in chickens and turkeys for coccidiosis control
The body weights of males and females showed a steady growth tendency and no significant statistical difference was noted at each time points
Our results showed that daily administration of ethanamizuril at dose of 60 mg/ kg are typical safe in Beagle dog

Summary

Introduction

Triazine coccidiostats are widely used in chickens and turkeys for coccidiosis control. Ethanamizuril is a novel triazine compound that exhibits anticoccidial activity in poultry. Coccidiosis is a detrimental disease of the intestinal tract of animals caused by several species of Eimeria protozoa. In the commercial broiler industry, Eimeria protozoan is parasitizing on the intestinal epithelium of chicken, which led to considerable impairment of growth and feed utilization, and resulted in significant impact on mortality and morbidity. Lack of safe and effective coccidiosis vaccines, commercial synthetic anticoccidial drug has been used as the main strategy for control of coccidiosis since the late 1940s [3]. The widely use of triazine coccidiostats have generated resistance to the genus Eimeria in recent years [5, 6]. No cross-resistance was observed in triazine coccidiostats [5]

Methods

Results

Discussion

Conclusion