BdCIPK31, a Calcineurin B-Like Protein-Interacting Protein Kinase, Regulates Plant Response to Drought and Salt Stress.

Qingchen Luo,Ruibin Wang,Fan Zhang,Yang Zhang,Shiyi Zhou,Qiuhui Wei,Jialu Feng,Guangxiao Yang,Guangyuan He,Yuan He

doi:10.3389/fpls.2017.01184

Abstract

Calcineurin B-like protein interacting protein kinases (CIPKs) are vital elements in plant abiotic stress signaling pathways. However, the functional mechanism of CIPKs has not been understood clearly, especially in Brachypodium distachyon, a new monocot model plant. In this study, BdCIPK31, a CIPK gene from B. distachyon was characterized. BdCIPK31 was downregulated by polyethylene glycol, NaCl, H2O2, and abscisic acid (ABA) treatments. Transgenic tobacco plants overexpressing BdCIPK31 presented improved drought and salt tolerance, and displayed hypersensitive response to exogenous ABA. Further investigations revealed that BdCIPK31 functioned positively in ABA-mediated stomatal closure, and transgenic tobacco exhibited reduced water loss under dehydration conditions compared with the controls. BdCIPK31 also affected Na+/K+ homeostasis and root K+ loss, which contributed to maintain intracellular ion homeostasis under salt conditions. Moreover, the reactive oxygen species scavenging system and osmolyte accumulation were enhanced by BdCIPK31 overexpression, which were conducive for alleviating oxidative and osmotic damages. Additionally, overexpression of BdCIPK31 could elevate several stress-associated gene expressions under stress conditions. In conclusion, BdCIPK31 functions positively to drought and salt stress through ABA signaling pathway. Overexpressing BdCIPK31 functions in stomatal closure, ion homeostasis, ROS scavenging, osmolyte biosynthesis, and transcriptional regulation of stress-related genes.

Highlights

Abiotic stress severely constrains the growth and development of plants, causes crop yield losses and even death (Bartels and Sunkar, 2005)
The cDNA of a CBL-interacting protein kinase (CIPK) gene was amplified by RT-PCR using mRNA isolated from B. distachyon seedlings
Multiple sequence alignment with these homologs indicated that BdCIPK31 contained all the typical features of CIPK domains, namely, an activation loop, NAF/FISL motif, and a protein–phosphatase interaction domain (Supplementary Figure S2)

Summary

Introduction

Abiotic stress severely constrains the growth and development of plants, causes crop yield losses and even death (Bartels and Sunkar, 2005). Plants have developed a complex mechanism to prevent damages caused by environmental changes. Ca2+ sensors detect the change in [Ca2+]cyt and transduce the signal to sensor responder proteins (Yu et al, 2014). During the past 20 years, multiple families of plant Ca2+ sensors and responders are identified, including calmodulins, CDPKs, CBLs, and CIPKs (Asano et al, 2005; Luan, 2009). Among these proteins, CBL-CIPK networks and CDPKs are unique to higher plants (Liu and Zhu, 1998; Kudla et al, 1999)

Methods

Results

Conclusion