Bcl6 for identification of germinal centres in salivary gland biopsies in primary Sjögren's syndrome.

Uzma Nakshbandi,Arjan Vissink,Hendrika Bootsma,Bert Van Der Vegt,Erlin A Haacke,Frans G M Kroese,Fred K L Spijkervet

doi:10.1111/odi.13276

Uzma Nakshbandi, Arjan Vissink + Show 5 more

Open Access

https://doi.org/10.1111/odi.13276

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Abstract

Histopathological assessment of salivary gland biopsies is an important element of the diagnostic work-up of Sjogren's syndrome (SS) (Fox, 2017; Kroese et al., 2018). Microscopic evaluation of salivary glands of primary SS (pSS) patients reveals characteristic periductal lymphocytic infiltrates (foci), which mainly consist of T- and B-lymphocytes, as well as a variety of non-lymphoid cells, including dendritic cells and macrophages. Over time, these infiltrates may become organised to ectopic lymphoid tissue with T/B cell compartmentalisation, presence of CD21+ follicular dendritic cell (FDC) networks and high endothelial venules (Fisher et al., 2017; Kroese et al., 2014; Kroese et al., 2018; Salomonsson et al., 2003).

Highlights

These infiltrates may become organised to ectopic lymphoid tissue with T/B cell compartmentalisation, presence of CD21+ follicular dendritic cell (FDC) networks and high endothelial venules (Fisher et al, 2017; Kroese et al, 2014, 2018; Salomonsson et al, 2003)
After staining for Bcl6, we showed that only 18% (9/50) of the labial gland foci with CD21+FDC networks and 32% (22/69) of the parotid gland foci with CD21+FDC networks comprised Bcl6+Germinal centres (GCs)
We observed a significant correlation between CD21+FDC networks/mm2 in parotid and labial salivary gland biopsies (Figure 1d, r = .60, p = .001), indicating comparability in lymphoid organisation at these two anatomical sites

Summary

University of Groningen

Bcl for identification of germinal centres in salivary gland biopsies in primary Sjogren's syndrome Nakshbandi, Uzma; Haacke, Erlin A.; Bootsma, Hendrika; Vissink, Arjan; Spijkervet, Fred K. Bcl for identification of germinal centres in salivary gland biopsies in primary Sjogren's syndrome. More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverneamendment. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. | | Received: 7 October 2019 Revised: November 2019 Accepted: December 2019

SHORT COMMUNICATION

Histopathological parameters of the parotid gland

Findings

Number of biopsies with foci