B-box Proteins in Arachis duranensis: Genome-Wide Characterization and Expression Profiles Analysis

Hanqi Jin,Mengge Xing,Chunmei Cai,Shuai Li

doi:10.3390/agronomy10010023

Hanqi Jin, Mengge Xing + Show 2 more

Open Access

https://doi.org/10.3390/agronomy10010023

Copy DOI

Journal: Agronomy	Publication Date: Dec 23, 2019
Citations: 19	License type: CC BY 4.0

Affiliation: Qingdao Agricultural University

Abstract

B-box (BBX) proteins are important factors involved in plant growth and developmental regulation, and they have been identified in many species. However, information on the characteristics and transcription patterns of BBX genes in wild peanut are limited. In this study, we identified and characterized 24 BBX genes from a wild peanut, Arachis duranensis. Many characteristics were analyzed, including chromosomal locations, phylogenetic relationships, and gene structures. Arachis duranensis B-box (AdBBX) proteins were grouped into five classes based on the diversity of their conserved domains: I (3 genes), II (4 genes), III (4 genes), IV (9 genes), and V (4 genes). Fifteen distinct motifs were found in the 24 AdBBX proteins. Duplication analysis revealed the presence of two interchromosomal duplicated gene pairs, from group II and IV. In addition, 95 kinds of cis-acting elements were found in the genes’ promoter regions, 53 of which received putative functional predictions. The numbers and types of cis-acting elements varied among different AdBBX promoters, and, as a result, AdBBX genes exhibited distinct expression patterns in different tissues. Transcriptional profiling combined with synteny analysis suggests that AdBBX8 may be a key factor involved in flowering time regulation. Our study will provide essential information for further functional investigation of AdBBX genes.

Highlights

IntroductionTranscription factors are essential components of signal transduction pathways in plants
Transcription factors are essential components of signal transduction pathways in plants.They often work as activators or suppressors, binding cis-acting elements in target promoter regions to regulate downstream gene expression [1,2]
To identify BBX genes in A. duranensis, we used the amino acid sequences of the BBX conserved domain (PF00643) and 32 Arabidopsis BBXs as blast queries against the peanut genome database

Summary

Introduction

Transcription factors are essential components of signal transduction pathways in plants. They often work as activators or suppressors, binding cis-acting elements in target promoter regions to regulate downstream gene expression [1,2]. Various transcription factor families are found in plants, which are involved in many different response pathways. Among these gene families, zinc-finger transcription factors, possess a conserved domain that can bind metal ions like zinc and interact with. A subgroup of zinc-finger proteins containing B-box (BBX) conserved domains, which are thought to be involved in protein–protein interactions, is designated as the BBX family and is highly conserved across all multicellular species [4,7,8,9]

Methods

Results

Conclusion