Bazı rezene popülasyonlarının (Foeniculum vulgare Mill.) ISSR markörlerine dayalı moleküler varyasyonu

Abstract

This research was conducted to determine the molecular variation between different fennel populations. In this research, six fennel populations, of which two were collected from natural flora, were used as a material. The molecular characterization of the populations was performed using six ISSR (Inter simple sequence repeat) primers. A total of 57 bands were obtained, of which 48 were evaluated as polymorphic. The average polymorphism percentage was 85%. The PIC values changed from 0.33 (ISSR-16) to 0.43 (ISSR-11) with an average of 0.37. The average RP (Resolving Power) value of the primers was found as 4.17, and the highest value was obtained from the ISSR-4 (6.33) primer. The mean EMR value was 6.78, and the highest value was obtained from the ISSR-4 (10.29) primer. The mean MI (Marker Index) value was estimated to be 2.47, with the highest value similarly observed in the ISSR-4 (3.67) primer. ISSR-4 was the most prominent primer in terms of these three parameters and the maximum total and polymorphic bands. The similarity values of the fennel populations were found to be between 0.30 and 0.95. The Mental test conducted between Jaccard's similarity matrices and the cluster result revealed the r value as 0.99, showing a very good correlation between the ISSR-based similarity matrix and the cluster. The populations of Burdur, Denizli, Isparta and Izmir, which were cultivated and traded, were genetically similar in terms of the ISSR primers examined, whereas Manisa Yunt Mountain and Odemis populations collected from natural flora were in separate groups.