Bazı İlaç ve Metallerin Keçi (Capra aegagrus hircus) Böbreğinde Aldoz Redüktaz ve Sorbitol Dehidrogenaz Enzimleri Üzerine Etkilerinin Araştırılması

Abstract

The effects of some antibiotics and metals on goat (Capra aegagrus hircus) kidney aldose reductase (AR) and sorbitol dehydrogenase (SDH) activities were examined. For drugs and metals that exhibit inhibitory effect, Ki constants were calculated and inhibition types were determined by using Lineweaver-Burk curves. In our study, the highest inhibition showed ceftriaxone antibiotic in AR enzyme with an IC50 value of 0.0274 mM. More potent AR inhibitors can be synthesized from the ceftriaxone compound. This compound is followed by amikacin sulfate and ciproflaksasin compounds with IC50 values of 0.061 and 0.25 mM, respectively. The highest inhibition of metals showed Co+2 ion with IC50 value 0.000445 mM. This metal is followed by Fe+2 and Zn+2 with IC50 values of 0.0286 and 0.084 mM, respectively. In SDH enzyme, the highest inhibition showed rifamycin sodium antibiotic with an IC50 value of 0.016 mM. More potent SDH inhibitors can be synthesized from the rifamycin sodium compound. This compound is followed by ceftriaxone and cefuroxime compounds with IC50 values of 0.025 and 0.16 mM, respectively. The highest inhibition of metals showed Co+2 ion with IC50 value 0.00044 mM. This metal is followed by Fe+2 and Zn+2 with IC50 values of 0.009 and 0.16 mM, respectively.