Bazı Entomopatojen Fungusların Leptinotarsa decemlineata Say'nin Farklı Biyolojik Dönemleri Üzerindeki Ölümcül Etkilerinin Belirlenmesi

Abstract

The lethal effects of Beauveria bassiana BIM-001 (1x106 spore mL-1, 1x107 spore mL-1, 1x108 spore mL-1), Fusarium subglutinans 12A (obtained from cotton aphid in Adana-Karataş) and the commercial preparation of 1.5% B. bassiana strain Bb-1 (250 mL da; 1x108 spore mL-1) (Nostalgist SL) on Leptinotarsa decemlineata were investigated. The isolates were applied to the eggs, larvae (1st, 2nd, 3rd, and 4th larval stages) and adults of L. decemlineata by spraying in laboratory conditions (25 ± 1°C, 60 ± 10% relative humidity and 16:8 [L: D] photoperiod). The observations to determine the lethal effect of isolates were performed up to 9 days for the eggs and larvae, and 21 days for the adults of L. decemlineata following the first spore suspension application. In the experiments conducted on the larval stages, it was determined that the mortality rates due to all entomopathogenic fungus isolates and at different concentrations were significantly different from the mortality rate of the control. The effect of all spore concentrations of B. bassiana BIM-001 was found significantly higher than other treatments and the mortality rates were 100% for all larval stages (P ˂0.05). In addition, the mortality rates were between 4 and 16% for adults across all treatment groups except for the control. Moreover, it has been determined that each three spore concentrations of B. bassiana BIM-001 also suppressed the egg hatching of L.decemlineata by 55 to 60%