Batch-mode stimulation of hydrocarbons biodegradation in freshwater sediments from historically contaminated Alūksne lake

Abstract

Historical contamination of freshwater lakes with hydrocarbons (HC) due to anthropogenic activities represents a serious problem worldwide. This study was focused on hydrocarbons-contaminated sediments sampled in Lake Alūksne of glacial origin in Northeast Latvia. The batch experiments were aimed at evaluating the effect of bio-stimulation and bioaugmentation on the biodegradation of hydrocarbons in lake sediments (LS), as well as changes in microbial community structure and metabolic activity. The sediments were sampled from two points of the lake, 4–5 m and 8 m depth, respectively. These samples slightly differed by colour, count of diatoms, microbial respiration intensity and colour intensity of 2,6- dichlorophenolindophenol. Nevertheless, the trend in biodegradation activity was similar for both LS samples. The concentration of HC in LS during the 32-day incubation decreased in average from 465 mg/kg to 165 mg/kg and 117.5 mg/kg in the LS amended with nutrients and nutrients+microbial community, respectively. Different treatment types of LS resulted in differences in microbial respiration and HC-degrading activity. The Shotgun sequencing has revealed the main phyla present in the intact LS being Proteobacteria (48.8%), Actinobacteria (24.4%), Firmicutes (10.4%) and Bacteroidetes (5.0%). Incubation of LS for 32 days resulted in increasing abundance of Proteobacteria from 48.8% in the raw LS to 58–62%, mainly due to the increase of Betaproteobacteria. The functional annotation of gene families revealed that the most abundant gene families were associated with ATP binding, metal ion, magnesium ion, sulfur cluster, zinc ion binding, DNA binding and other essential components for cell functioning. The Shannon biodiversity index of culturable microorganisms in EcoPlates™ ranged from 2.28 to 2.85. The data obtained in this study indicated that the suggested approach is a potent remediation technology for further ex situ scaling up.