Abstract
Simple and sensitive batch and Flow-injection spectrophotometric methods for the determination of Procaine HCl in pure form and in injections were proposed. These methods were based on a diazotization reaction of procaine HCl with sodium nitrite and hydrochloric acid to form diazonium salt, which is coupled with chromatropic acid in alkaline medium to form an intense pink water-soluble dye that is stable and has a maximum absorption at 508 nm. A graphs of absorbance versus concentration show that Beer’s law is obeyed over the concentration range of 1-40 and 5-400 µg.ml-1 of Procaine HCl, with detection limits of 0.874 and 3.75 µg.ml-1 of Procaine HCl for batch and FIA methods respectively. The FIA average sample throughput was 70 h-1. All different chemical and physical experimental parameters that affecting on the development and stability of the colored product were carefully studied and the proposed methods were applied satisfactorily for the determination of Procaine HCl in an injections samples using the standard addition method.
Highlights
IntroductionProcaine is a local anesthetic can be used either alone or with penicillin as an antibacterial drug its chemical name is2-diethyl aminoethyl-4-aminobenzoate hydrochloride and its formula C13H20N2O2, with molecular weight of 272.8 the structure of procaine HCl is [1]: Procaine was first synthesized in 1905, it quickly replaced cocaine because it is easier to synthesize and sterilize, shorter duration of action, non addictive and it has four to six times less toxic than cocaine, procaine, like other local anesthetics such as tetracaine acts as a nerve block, halting the generation and conduction of nerve impulses signal pain, it is used in obstetrics and some times for relief pain in the lower back and tooth extraction [2].Several methods have been reported for the determination of procaine HCl include High performance liquid chromatography [3,4], differential-plus voltammetry and electrochemichal analysis [5,6,7,8], electrophoresis [9,10], atomic absorption [11], ion association titration [12], gas chromatography [13], fluorimetry [14,15,16], sequential injection analysis [17,18], flow injection analysis [19,20], chemiluminsense [21], colorimetry and spectrophotometric methods [22-28].The present study describes the development of batch and FIA methods based on diazotiazation and coupling reaction between diazotizedProcaine HCl and chromatropic acid reagent in alkaline medium
The factors that affecting on the sensitivity and stability of the colored product resulting from the diazonium reaction between diazotized Procaine HCl and chromatropic acid in alkaline medium were carefully studied
A 1:1 mole ratio of Procaine HCl to sodium nitrite of (1.83x10-3M) was used in order to prevent the effect of excess of sodium nitrite
Summary
Procaine is a local anesthetic can be used either alone or with penicillin as an antibacterial drug its chemical name is2-diethyl aminoethyl-4-aminobenzoate hydrochloride and its formula C13H20N2O2, with molecular weight of 272.8 the structure of procaine HCl is [1]: Procaine was first synthesized in 1905, it quickly replaced cocaine because it is easier to synthesize and sterilize, shorter duration of action, non addictive and it has four to six times less toxic than cocaine, procaine, like other local anesthetics such as tetracaine acts as a nerve block, halting the generation and conduction of nerve impulses signal pain, it is used in obstetrics and some times for relief pain in the lower back and tooth extraction [2].Several methods have been reported for the determination of procaine HCl include High performance liquid chromatography [3,4], differential-plus voltammetry and electrochemichal analysis [5,6,7,8], electrophoresis [9,10], atomic absorption [11], ion association titration [12], gas chromatography [13], fluorimetry [14,15,16], sequential injection analysis [17,18], flow injection analysis [19,20], chemiluminsense [21], colorimetry and spectrophotometric methods [22-28].The present study describes the development of batch and FIA methods based on diazotiazation and coupling reaction between diazotizedProcaine HCl and chromatropic acid reagent in alkaline medium. Aminobenzoate hydrochloride and its formula C13H20N2O2, with molecular weight of 272.8 the structure of procaine HCl is [1]: Procaine was first synthesized in 1905, it quickly replaced cocaine because it is easier to synthesize and sterilize, shorter duration of action, non addictive and it has four to six times less toxic than cocaine, procaine, like other local anesthetics such as tetracaine acts as a nerve block, halting the generation and conduction of nerve impulses signal pain, it is used in obstetrics and some times for relief pain in the lower back and tooth extraction [2].Several methods have been reported for the determination of procaine HCl include High performance liquid chromatography [3,4], differential-plus voltammetry and electrochemichal analysis [5,6,7,8], electrophoresis [9,10], atomic absorption [11], ion association titration [12], gas chromatography [13], fluorimetry [14,15,16], sequential injection analysis [17,18], flow injection analysis [19,20], chemiluminsense [21], colorimetry and spectrophotometric methods [22-28]. The reaction can be carried out in batch and FIA and the two approaches are compared
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
