Basin-scale spatio-temporal variability and control of phytoplankton photosynthesis in the Baltic Sea: The first multiwavelength fast repetition rate fluorescence study operated on a ship-of-opportunity

Abstract

This study presents the results of the first field application of a flow-through multi-wavelength Fast Repetition Rate fluorometer (FRRF) equipped with two excitation channels (458 and 593nm). This device aims to improve the measurement of mixed cyanobacteria and algae community's photosynthetic parameters and was designed to be easily incorporated into existing ferrybox systems. We present a spatiotemporal analysis of the maximum photochemical efficiency (Fv/Fm) and functional absorption cross section (σPSII) recorded from April to August 2014 on a ship-of-opportunity commuting twice per week between Helsinki (Finland) and Travemünde (Germany). Temporal variations of Fv/Fm and σPSII differed between areas of the Baltic Sea. However, even though the Baltic Sea is characterized by several physico-chemical gradients, no gradient was observed in Fv/Fm and σPSII spatial distribution suggesting complex interactions between biotic and abiotic controls. σPSII was sensitive to phytoplankton seasonal succession and thus differed according to the wavelength used to excite photosystems II (PSII) pigments. This was particularly true in summer when high σPSII(593) values were observed later and longer than high σPSII(458) values, reflecting the role of cyanobacteria in photosynthetic light uptake measured at community scale. In contrast, Fv/Fm variations were similar after excitation at 458nm or 593nm suggesting that the adjustment of Fv/Fm in response to environmental factors was similar for the different groups (algae vs. cyanobacteria) present within the phytoplankton community.