Abstract
BackgroundBasigin, which has four isoforms, has been demonstrated to be involved in progression of various human cancers. The aim of this study was to examine the prognostic value of basigin-2 protein expression in epithelial ovarian cancer. Furthermore, the function of basigin-2 in ovarian cancer was further investigated in cell culture models.MethodsImmunohistochemistry staining was performed to investigate basigin-2 expression in a total of 146 ovarian tissue specimens. Kaplan Meier analysis and Cox proportional hazards model were applied to assess the relationship between basigin-2 and progression-free survival (PFS) and overall survival (OS). Real-time PCR, RT-PCR and western blot were used to explore basigin-2, basigin-3 and basigin-4 expression in ovarian cancer cell lines and tissues. To evaluate possible contributions of basigin-2 to MMP secretion and cell migration and invasion, the overexpression vectors pcDNA3.1-basigin-2 and basigin-2 siRNA were transfected into HO-8910 and HO-8910 PM cells respectively.ResultsHigh basigin-2 expression was associated with lymph-vascular space involvement, lymph node metastasis and poor prognosis of epithelial ovarian cancer. Multivariate analyses indicated that basigin-2 positivity was an independent prognostic factor for PFS (P = 0.006) and OS (P = 0.019), respectively. Overexpression of basigin-2 increased the secretion of MMP-2/9 and cancer cell migration and invasion of HO-8910 cells, whereas knockdown of basigin-2 reduced active MMP-2/9 production, migration and invasion of HO-8910 PM cells.ConclusionsThe expression of basigin-2 might be an independent prognostic marker and basigin-2 inhibition would be a potential strategy for epithelial ovarian cancer patients, especially in inhibiting and preventing cancer cell invasion and metastasis.
Highlights
Basigin, which has four isoforms, has been demonstrated to be involved in progression of various human cancers
By using univariate Cox proportional analysis, basigin-2 expression was statistically correlated to progression-free survival (P < 0.001, Table 2) and overall survival (P = 0.002, Table 2)
Our results indicated that high basigin-2 expression was significantly correlated with lymph-vascular space involvement and lymph node metastasis, suggesting that its expression might be important for the acquirement of malignant potential in epithelial ovarian cancer
Summary
Basigin, which has four isoforms, has been demonstrated to be involved in progression of various human cancers. The aim of this study was to examine the prognostic value of basigin-2 protein expression in epithelial ovarian cancer. Ovarian cancer is the most lethal gynecologic malignancy among women in many countries [1]. Epithelial ovarian cancer is the most common ovarian cancer and accounts for approximately 70% of all ovarian malignant diseases. It is the sixth most common cancer and the fifth leading cause of cancer-related death in women worldwide [2]. Despite significant advances in surgery and chemotherapy over the last few decades, therapeutic failure and disease progression are still quite frequent [4,5]. There is an urgent requirement for new biomarkers for ovarian cancer, so as to develop better early diagnostic and therapeutic strategies
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