Abstract
Flow cytometry (FC) has become an important component in the diagnosis and monitoring of patients with a diverse array of diseases. While the basic principals underlying FC remain relatively unchanged, the technology and array of available reagents have continued to evolve, thus expanding the list of applications in laboratory medicine. This review provides an overview of the basic theory of FC including instrumentation, sample processing, and analysis. Additionally, current and future clinical applications of FC are discussed. Among these, applications of FC in solid organ and hematopoeitic stem-cell transplantation, transfusion medicine, hematopathology, and infectious diseases are presented. Finally, applications employed primarily in clinical research are presented in anticipation of their eventual use in the clinical laboratory setting.
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