Abstract
In order to develop a sensitive and precise chemical bioanalysis of benzylpenicillin in biological fluids, basic studies on benzylpenicillin are presented. These studies include pH dependent stability, extraction in aqueous ethylacetate systems in different pH and buffer compositions. A pre-column derivatisation LC method for the detection of benzylpenicillin also is described. The derivatisation of the sample is performed by the β-lactam ring specific formation of the mercuric(II)mercaptide of the penicillenic acid in the presence of imidazole. The UV spectral properties of the derivative are utilised for detection. The chromatographic conditions are optimised with reference to the pH, the methanol, the imidazole and the mercury(II)chloride content of the mobile phase as well as the column temperature.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
