Abstract
An alternative method for simultaneous baseline separation of α and β-acids homologues and isomers in hop by CD-MEKC with UV detection was proposed. The optimized background electrolyte was composed of 30mmol/L sodium tetraborate solution, 45mmol/L sodium dodecyl sulfate, 20mmol/L β-cyclodextrin and 10% v/v acetonitrile. The instrumental conditions were evaluated by using a 33 Box-Benhken experimental design. In order to demonstrate the applicability of the method, 21 hop samples from different varieties were analyzed. The repeatability intra- and interday tests were performed and relative standard deviations lower than 7% for area and migration times were observed. The present method comprehended 8min analysis time and revealed to be faster and more efficient when compared to previous reports from literature.
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