Abstract
Cyanine derivatives, the raw materials of classic near-infrared fluorescence probes, have been often used and developed to study imaging under different spectroscopes. In this paper, 4-carboxyphenylhydrazine hydrochloride, methyl iodide and p-hydroxybenzaldehyde were used as starting materials. After three-step reaction, compound (E)-5-carboxy-2-(4-hydroxy-styrene)-1, 3, 3-Trimethyl-3H-indole iodide (Compound 1). The intermediate and final products have been characterized by hydrogen spectroscopy, carbon spectroscopy and high-resolution mass spectrometry. This article mainly includes the following two parts: 1. Synthesis of (E)-5-carboxy-2-(4-hydroxy-styrene)-1, 3, 3-trimethyl-3H-indole iodide 2. The characterization part of the final product and the intermediate product is mainly hydrogen spectrum, carbon spectrum and mass spectrum.
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