Abstract
According to French Paradox, red wine was famous for the potential effects on coronary heart disease (CHD), but the specific compounds against CHD were unclear. Therefore, screening and characterization of bioactive compounds from red wine was extremely necessary. In this paper, the multi-activity integrated strategy was developed and validated to screen, identify and quantify active compounds from red wine by using ultra high performance liquid chromatography-fraction collector (UHPLC-FC), ultra fast liquid chromatography-quadrupole-time-of-flight/mass spectrometry (UFLC-Q-TOF/MS) and bioactive analysis. UHPLC-FC was employed to separate and collect the components from red wine, which was further identified by UFLC-Q-TOF/MS to acquire their structural information. Furthermore, the active fractions were tested for antioxidant activity, inhibitory activity against thrombin and lipase activities in vitro by the activity screening kit. As the results, there were 37 fractions had antioxidant activity, 22 fractions had thrombin inhibitory activity and 28 fractions had lipase inhibitory activity. Finally, 77 active components from red wine were screened and 12 ingredients out of them were selected for quantification based on the integration of multi-activity. Collectively, the multi-activity integrated strategy was helpful for the rapid and effective discovery of bioactive components, which provided reference for exploring the health care function of food.
Highlights
As a nutritious drink recommended by World Health Organization (WHO), red wine was widely recognized for its role in health care
Modern pharmacological studies showed that red wine had the function of antioxidant, anticoagulant and lipidlowering [1,2], which can prevent cardiovascular and cerebrovascular diseases such as coronary heart disease (CHD) [3,4]
It was widely believed that the mechanisms of CHD were the result of the interaction of many complex factors, among them the “response to injury hypothesis” had been recognized by researchers [5,6], which stated that the initial damage was the arterial endothelium, further leading to endothelial dysfunction [7]
Summary
As a nutritious drink recommended by World Health Organization (WHO), red wine was widely recognized for its role in health care. Modern pharmacological studies showed that red wine had the function of antioxidant, anticoagulant and lipidlowering [1,2], which can prevent cardiovascular and cerebrovascular diseases such as coronary heart disease (CHD) [3,4]. There were three main methods to treat CHD including pharmacotherapy, interventional therapy and operation [9,10]. These methods hold the potential effects for CHD, these methods would lead some side effects such as common complications after coronary intervention included bleeding, pulmonary embolism, infection and so on. Prevention CHD has become the trend of medical development
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