Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions

Inas A Rasheed

doi:10.47723/kcmj.v13i2.95

Abstract

 Background: The diagnosis of prostatic pathology may be of challenging , as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from malignant prostatic lesions. Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions. Type of the study: A retro-spective study. Methods: 115cases of paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63. Results : basal cells were detected in cases of BPH , and HG-PIN , and absent in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2% for BPH and 57.1% and 55.6% for HG-PIN immunostained with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively with combined using of both markers .Conclusion : Combination of both basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions.

Highlights

The diagnosis of prostatic pathology may be of challenging, as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections
For BPH,negative benign glands (>2) were found in 71.6% and 38.2% of cases stained with 34BE12 and p63, respectively, but significantly reduced to 9.0% when combination of both markers (34BE12 and p63) were used (p=0.012), Fig(3)
The difference in immunoreactivity that seen between both factors may be due to susceptibility of 34BE12 to formalin fixation

Summary

Introduction

The diagnosis of prostatic pathology may be of challenging , as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections. Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from malignant prostatic lesions. Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions. Methods: 115cases of paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63. Negative benign glands(>2) were found in 71.6% and 38.2% for BPH and 57.1% and 55.6% for HG-PIN immunostained with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-

Objectives

Results

Discussion

Conclusion