Abstract
<i>Bartonella</i>Species in Raccoons and Feral Cats, Georgia, USA
Highlights
DNA was extracted from blood by using a commercial DNA extraction kit (Quick-gDNA MiniPrep; Zymo Research Corp., Orange, CA, USA)
B. koehlerae was amplified from 1 feral cat sample and 1 raccoon sample
Two feral cat samples were identified as containing B. clarridgeiae and showed 98% and 100% sequence homology with a B. clarridgeiae sequence
Summary
DNA was extracted from blood by using a commercial DNA extraction kit (Quick-gDNA MiniPrep; Zymo Research Corp., Orange, CA, USA). Intergenic spacer sequences from raccoon isolates were aligned with reported Bartonella species sequences in GenBank by using the ClustalW algorithm [6]. Data were resampled 1,000 times to generate bootstrap values by using MEGA5 [7]. Of 74 samples analyzed (37 raccoon, 37 feral cat), 16 (43%) raccoon samples and 18 (48%) feral cat samples were positive for Bartonella spp. by PCR.
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