Abstract
<I>Staphylococcus epidermidis</I>, commonly found on the human skin, may contaminate human milk. High-pressure pasteurisation of human milk under normal temperature preserves the majority of its protective agents. The objective of this study was to acquire baroinactivation data and develop a model for model solutions of pH = 6.4 to 7.2 and water activity a<sub>w</sub> = 0.99, in which baroinactivation of <I>Staphylococcus epidermidis</I> takes place. Decontamination data manifested exponential kinetics and the resulting model was described by the following equations: D<sub>p</sub> = D<sub>p, ref</sub> × 10 <sup>(Pref – P)/Z</sup>, Z = –123.90 pH<sup>2</sup> + 1635.54 pH – 5210.49; D<sub>p, ref</sub> = –8.89 pH<sup>2</sup> + 121.02 pH – 408.34. The developed model was verified using pasteurised human milk and UHT-treated skimmed cow milk. The agreement between the experimental data and model-based prediction was very good for human milk. It was proved that the application of a pressure of 350 MPa for 10 min decreased the concentration of the working suspension of <I>S. epidermidis</I> in the model substrate by a minimum of five orders.
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