Barley HvHMA1 is a heavy metal pump involved in mobilizing organellar Zn and Cu and plays a role in metal loading into grains.

Maria Dalgaard Mikkelsen,Eva Vincze,Søren Borg,Michaela Schiller,Preben Bach Holm,Pai Pedas,Lone Baekgaard,Jan K Schjoerring,Rebecca F Mills,Annette Møller,Michael G Palmgren,Lorraine E Williams

doi:10.1371/journal.pone.0049027

Maria Dalgaard Mikkelsen, Eva Vincze + Show 10 more

Open Access

https://doi.org/10.1371/journal.pone.0049027

Copy DOI

Abstract

Heavy metal transporters belonging to the P1B-ATPase subfamily of P-type ATPases are key players in cellular heavy metal homeostasis. Heavy metal transporters belonging to the P1B-ATPase subfamily of P-type ATPases are key players in cellular heavy metal homeostasis. In this study we investigated the properties of HvHMA1, which is a barley orthologue of Arabidopsis thaliana AtHMA1 localized to the chloroplast envelope. HvHMA1 was localized to the periphery of chloroplast of leaves and in intracellular compartments of grain aleurone cells. HvHMA1 expression was significantly higher in grains compared to leaves. In leaves, HvHMA1 expression was moderately induced by Zn deficiency, but reduced by toxic levels of Zn, Cu and Cd. Isolated barley chloroplasts exported Zn and Cu when supplied with Mg-ATP and this transport was inhibited by the AtHMA1 inhibitor thapsigargin. Down-regulation of HvHMA1 by RNA interference did not have an effect on foliar Zn and Cu contents but resulted in a significant increase in grain Zn and Cu content. Heterologous expression of HvHMA1 in heavy metal-sensitive yeast strains increased their sensitivity to Zn, but also to Cu, Co, Cd, Ca, Mn, and Fe. Based on these results, we suggest that HvHMA1 is a broad-specificity exporter of metals from chloroplasts and serve as a scavenging mechanism for mobilizing plastid Zn and Cu when cells become deficient in these elements. In grains, HvHMA1 might be involved in mobilizing Zn and Cu from the aleurone cells during grain filling and germination.

Highlights

In all cells, Zn and Cu are essential heavy metal micronutrients in excess they can be toxic [1,2,3,4,5]
We suggest that HvHMA1, which is highly expressed in the endosperm, is involved in Zn and Cu homeostasis during grain filling, a function that is important for controlling the total Zn and Cu content in mature grains
Sequence Analysis of HvHMA1, a Close Orthologue of AtHMA1. It was previously found by sequence analysis of expressed sequence tags (ESTs) that barley contains a P1B-ATPase with homology to rice OsHMA1 and A. thaliana AtHMA1 [29]

Summary

Introduction

Zn and Cu are essential heavy metal micronutrients in excess they can be toxic [1,2,3,4,5] They function as cofactors, either as structural stabilizers such as transcription factors, or as functional components of proteins, like in the active sites of enzymes [3,6,7]. Because Zn and Cu are essential in all cells but toxic in excess, their transport and homeostasis are tightly regulated [12,13,14] In plants, these heavy metals are taken up from the soil through the roots and subsequently exported from xylem parenchyma cells into xylem vessels responsible for long distance transport to the shoot [15,16,17]. Export of positively charged heavy metal ions from maternal cells has to be active in order to overcome the positive-outside membrane potential created by plasma membrane H+-ATPases [17,24,25]

Methods

Results

Conclusion