Abstract
Abstract The effect of temperature on efficiency in micellar liquid chromatography (MLC) has been investigated using an SDS micellar mobile phase and a C18 stationary phase. Application of the Knox equation to plate count data yielded crucial information about band broadening in MLC. The improvement in chromatographic efficiency with temperature is due to a decrease in both the A (flow anisotropy) and C (stationary phase mass transfer) terms of the Knox equation. The decrease in the A term can be attributed to a shift in the position of the equilibrium of the solute away from the micelle and towards the bulk solvent; the decrease in the C term with temperature can be explained in terms of surfactant adsorption which tends to increase both the thickness and viscosity of the stationary phase. By increasing the operating temperature of the column, less surfactant is adsorbed on the stationary phase. Due to concerns about the validity of the Knox equation to describe band broadening in MLC, it was necessary t...
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