Banana Lectin from Musa paradisiaca Is Mitogenic for Cow and Pig PBMC via IL-2 Pathway and ELF1

Roxane L Degroote,Marie-Christin Schilloks,Kristina J H Kleinwort,Barbara Amann,Sieglinde Hirmer,Cornelia A Deeg,Stefanie M Hauck,Franziska Stetter,Lucia Korbonits

doi:10.3390/immuno1030018

Roxane L Degroote, Marie-Christin Schilloks + Show 7 more

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https://doi.org/10.3390/immuno1030018

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Abstract

The aim of the study was to gain deeper insights in the potential of polyclonal stimulation of PBMC with banana lectin (BanLec) from Musa paradisiaca. BanLec induced a marked proliferative response in cow and pig PBMC, but was strongest in pigs, where it induced an even higher proliferation rate than Concanavalin A. Molecular processes associated with respective responses in porcine PBMC were examined with differential proteome analyses. Discovery proteomic experiments was applied to BanLec stimulated PBMC and cellular and secretome responses were analyzed with label free LC-MS/MS. In PBMC, 3955 proteins were identified. After polyclonal stimulation with BanLec, 459 proteins showed significantly changed abundance in PBMC. In respective PBMC secretomes, 2867 proteins were identified with 231 differentially expressed candidates as reaction to BanLec stimulation. The transcription factor “E74 like ETS transcription factor 1 (ELF1)” was solely enriched in BanLec stimulated PBMC. BanLec induced secretion of several immune regulators, amongst them positive regulators of activated T cell proliferation and Jak-STAT signaling pathway. Top changed immune proteins were CD226, CD27, IFNG, IL18, IL2, CXCL10, LAT, ICOS, IL2RA, LAG3, and CD300C. BanLec stimulates PBMC of cows and pigs polyclonally and induces IL2 pathway and further proinflammatory cytokines. Proteomics data are available via ProteomeXchange with identifier PXD027505.

Highlights

Lectins recognize cell-surface carbohydrates with high specificity and are important for many biological processes [1]
1A, blue bar), whereas stimulation with resulted in lowest of bovine PBMC with banana lectin (BanLec) for 48 h resulted in a marked proliferative proliferation rate
We observed a difference in overall proliferation capacity between cows and pigs in this study, which was greatest for BanLec: this mitogen caused a more than two-fold higher study, which was greatest for BanLec: this mitogen caused a more than two-fold higher proliferation rate in pig PBMC compared to PBMC from cows

Summary

Introduction

Lectins recognize cell-surface carbohydrates with high specificity and are important for many biological processes [1]. Mitogenic lectins can promote polyclonal stimulation in PBMC [3,4]. We showed that a lectin from the banana Musa paradisiaca highly stimulated in vitro proliferation of cow PBMC [5]. A subgroup of cows reacted with hyperstimulation to classical polyclonal stimulants like pokeweed mitogen [6,7], concanavalin. A [4], and to BanLec from M. paradisiaca [5] This banana lectin was the first lectin isolated from the large family of Musa [8]. Bananas are a large family, comprising dessert bananas and plantains [9]. Both belong to the large and perpetually increasing family of Musaceae [9,10]. Lectin activity is restricted to certain varieties of banana [11]. These banana lectins belong to the mannose-specific jacalin-related

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