Baked Corn (Zea mays L.) and Cooked Common Bean (Phaseolus vulgaris L.) Snack Consumption Reduced Inflammation and Upregulated NRF2 and SOD2 in Chronic Colitis In Vivo

Baked corn (Zea mays L.) and cooked common bean (Phaseolus vulgaris L.) chips improved enzymatic biomarkers and alleviated inflammation during chronic colitis in vivo (P06-063-19)

Consumption of a baked corn and bean snack reduced chronic colitis inflammation in CD-1 mice via downregulation of IL-1 receptor, TLR, and TNF-α associated pathways

Cyclic administrations of dextran sulphate sodium (DSS) alternating with distilled water usually induce chronic colitis after a few weeks. In order to obtain stable chronic colitis (without recovery or relapse) in a few days, a new continuous DSS treatment was tested and characterized. Short-chain fatty acids (SCFAs), which remain poorly documented in experimental colitis, were also investigated. Thirty-six Sprague-Dawley rats were treated with 5% DSS for 7 days (DI) followed by 3% DSS for 7 days (DM) or 14 days (DF). Control rats received only water. Inflammatory injuries in the caecum and the colon were assessed by macroscopic (colon length, caecum weight, damages score) and histological parameters. SCFAs (acetate, propionate, butyrate) were quantified individually in caecal, proximal and distal contents. Macroscopic and histological observations revealed that this continuous DSS treatment induced acute inflammation (DI) followed rapidly by chronic active colitis. The latter was uncommonly predominant in the caecum and the distal colon, and was also associated with some fermentative disturbances. Caecal SCFA concentrations decreased with DSS at DI and DM. The molar ratio of caecal butyrate increased with DSS. Acetate decreased in the colon while propionate increased. This new DSS treatment is able to induce in a few days stable chronic inflammation with caecal and distal predominant injuries, and mild fermentative caeco-colonic alterations. This model could contribute to the study of potential anti-inflammatory effects of prebiotics.

The third-largest land plant family, Fabaceae (Papilionaceae), includes trees, shrubs, and perennial or annual herbaceous plants containing both numerous beneficial constituents (e.g., proteins, carbohydrates, dietary fibre) and antinutrients (e.g., saponins, tannins, phytic acid, gossypol, lectins). The consumption of leguminous plants allows sports people to complete their requirements for nourishment but, on the other hand, it contributes to digestive system ailments. Therefore, the aim of the presented study was to review the experimental articles and patents referring to the application of common (kidney) bean (Phaseolus vulgaris L.)-based nutritional products for athletes. The survey of the literature was carried out according to PRISMA statements by browsing Scopus, PubMed and ISI Web of Science databases, as well as Google Scholar, Google Patents and Espacenet Patent Search engines using factorial combinations of the following keywords: (‘common bean’ or ‘kidney bean’ or ‘Phaseolus vulgaris’) and (‘athlete’ or ‘sport’) and (‘food’ or ‘nutrition’ or ‘diet’). Altogether, 84 patents issued in the years 1995–2023 were noted. The majority of patents were developed by research teams consisting of at least four authors representing scientists affiliated in the United States of America and China. The patents refer to the production of food ingredients, nutritional products, and compositions: (i) for relieving fatigue, enhancing endurance, and increasing muscle mass and strength, (ii) for maintaining physical and mental health, and (iii) for controlling body weight. Moreover, the analysis of 19 original articles indicated the substantial acceptability of meals containing the common bean. To summarize, the performed investigations demonstrate the considerable use of Phaseolus vulgaris in sport nutrition and the growing acceptance of this trend.

Epidermal Growth Factor Partially Restores Colonic Ion Transport Responses in Mouse Models of Chronic Colitis

Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders with important impact on global health. Prebiotic and probiotic strategies are thought to be useful in the context of experimental IBD. Here, we compared the effects of preventive versus therapeutic treatment with a high fiber diet (prebiotic) in combination or not with Bifidobacterium longum (probiotic) in a murine model of chronic colitis. Colitis was induced by adding dextran sulfate sodium (DSS) to drinking water for 6days (acute colitis) or for 5cycles of DSS (chronic colitis). Administration of the high fiber diet protected from acute colitis. Protection was optimal when diet was started 20days prior to DSS. A 5-day pretreatment with acetate, a short-chain fatty acid, provided partial protection against acute colitis. In chronic colitis, pretreatment with the high fiber diet attenuated clinical and inflammatory parameters of disease. However, when the treatment with the high fiber diet started after disease had been established, overall protection was minimal. Similarly, delayed treatment with acetate or B. longum did not provide any protection even when the probiotic was associated with the high fiber diet. Preventive use of a high fiber diet or acetate clearly protects mice against acute and chronic damage induced by DSS in mice. However, protection is lost when therapies are initiated after disease has been established. These results suggest that any therapy aimed at modifying the gut environment (e.g., prebiotic or probiotic strategies) should be given early in the course of disease.

Cancer is a leading cause of death worldwide with colorectal cancer (CRC) ranking as the third contributing to overall cancer mortality. Non-digestible compounds such as dietary fiber have been inversely associated with CRC in epidemiological in vivo and in vitro studies. In order to investigate the effect of fermentation products from a whole non-digestible fraction of common bean versus the short-chain fatty acid (SCFAs) on colon cancer cells, we evaluated the human gut microbiota fermented non-digestible fraction (hgm-FNDF) of cooked common bean (Phaseolus vulgaris L.) cultivar Negro 8025 and a synthetic mixture SCFAs, mimicking their concentration in the lethal concentration 50 (SCFA-LC50) of FNDF (hgm-FNDF-LC50), on the molecular changes in human colon adenocarcinoma cells (HT-29). Total mRNA from hgm-FNDF-LC50 and SCFA-LC50 treated HT-29 cells were used to perform qPCR arrays to determine the effect of the treatments on the transcriptional expression of 84 genes related to the p53-pathway. This study showed that both treatments inhibited cell proliferation in accordance with modulating RB1, CDC2, CDC25A, NFKB and E2F genes. Furthermore, we found an association between the induction of apoptosis and the modulation of APAF1, BID, CASP9, FASLG, TNFR10B and BCL2A genes. The results suggest a mechanism of action by which the fermentation of non-digestible compounds of common bean exert a beneficial effect better than the SCFA mixture by modulating the expression of antiproliferative and pro-apoptotic genes in HT-29 cells to a greater extent, supporting previous results on cell behavior, probably due to the participation of other compounds, such as phenolic fatty acids derivatives and biopetides.

Deletion of Mtgr1 Sensitizes the Colonic Epithelium to Dextran Sodium Sulfate–Induced Colitis

Intercropping maize (Zea mays L.) with common bean (Phaseolus vulgaris L.) is one of the predominant farming practices in eastern and southern Africa (ESA) for effective use of resources and continuous household food supply. The productivity of sole or intercropped crops is subject to variety, location, year, and their interaction. Therefore, the objective of this study was to determine the productivity of newly released common bean varieties NUA45 and SER83 under sole cropping and intercropping with a maize hybrid variety SC672 as a guide to large-scale production. Experiments were conducted at Chitedze Agricultural Research Station (13.85°S; 33.38°E) and Linthipe Extension Planning Area (12.06°S; 33.25°E) in 2019 and 2020 in Malawi using a factorial arrangement laid out in a randomized complete block design (RCBD) with four replications. The numbers of pods per plant (NPP) and seeds per pod (NSP), grain yield (GYD), and 100-seed weight were collected for common bean included, while GYD was recorded for maize. The main effects for genotype, location, year, and intercropping system were significant (p < 0.05) for GYD in common bean. The effects of the year and cropping system and location by intercropping system interaction were significant for maize GYD. The maize yield did not vary between sole cropped and intercropped systems. The total land equivalent ratios (LERs) for NUA45 and SER83 were 1.59 and 1.77, respectively. The LER-values showed a significant difference (p < 0.034), suggesting a considerably higher benefit of maize and common bean intercropping. Overall, intercropping maize with common bean rendered higher yields in the SER83/SC672 intercropping system than the sole crop in the study areas. Therefore, intra-row intercropping of the newly released common bean variety SER83 with a maize hybrid variety SC672 is recommended in the study area and other similar agro-ecologies for stable and sustainable production of both crops.

Common bean (Phaseolus vulgaris L.) is a major source of affordable, high-quality dietary protein that complements maize (Zea mays L.)-based diets. Traditional storage of these seeds in sacks and glass jars without treatment often leads to infestation by insect pests, such as Acanthoscelides obtectus and Sitophilus zeamais, causing significant grain losses. This study evaluated grain damage and weight loss after mixing biosilica with maize and common bean seeds, which were stored in various containers (glass jars, polyethylene, and polypropylene bags) over a six-month period, during which seed viability was also assessed. Although the initial moisture level (≤ 13%) was favourable for storage for both grain species, about half of the common bean cultivars (FEB 190 and NUV6) and two-thirds of the maize cultivars (CMS 8501 and CMS 8704) were adversely affected after prolonged storage without treatment for six months. As concerns the effects of the storage receptacle on the biosilica-treated seeds, the polyethylene bags and glass jars were more effective for common beans than for maize seeds after five months of storage. However, by the sixth month, the biosilica-containing jars were characterized by low insect perforation indices (≤ 50%) and rather high seed viability (> 80%) for both maize and common beans after six months of storage, highlighting the protective effect and storage loss reduction capacity of the biosilica. Hence, their application could help farmers improve safe grain storage over prolonged periods and eventually enhance their livelihoods and incomes.

Gallic acid (GA) is recognized for its purported antiinflammatory properties. GA has been demonstrated to prevent and alleviate the symptoms of chronic colitis through the modulation of the gut microbiota, improvement of the intestinal barrier, and reduction of inflammation. In order to determine the mechanism by which GA exerts its protective effect against chronic colitis, mice were induced by dextran sulfate sodium (DSS). The reduction in the disease activity index by 25% and the decrease in colon tissue damage indicated that 36 days of GA intervention alleviated chronic DSS-induced colitis symptoms. GA was observed to mitigate weight loss by 2.5% and the shortening of colon by 17.3%, and to diminish the expression of pivotal proteins within the TLR4/nuclear factor κB (NF-κB) signaling cascades, consequently lowering the generation of inflammatory cytokines. Furthermore, GA effectively corrected the gut microbiota imbalance, increased the content of short-chain fatty acids (SCFAs), which in turn suppressed inflammation, and enhanced tight junction protein expression, thereby strengthening the intestinal barrier. GA has the capacity to enhance the efficacy of chronic colitis through a multifaceted mechanism, influencing the gut microbiota, intestinal barrier function, and inflammatory processes. The findings highlight the potential of GA as a preventative strategy for chronic colitis.

IntroductionIron deficiency anaemia is common in inflammatory bowel disease (IBD). Iron supplementation may induce or exacerbate colitis in rats (APT 2001;15:1989–99). Dysbiosis is common in IBD and iron contributes to...

Context Utilising crop residues is an effective strategy to compensate for the shortage of common feed resources. Different processing methods can improve the nutritional value of these by-products. Aims This study aimed to investigate the effect of sodium hydroxide (NaOH, 50 g/kg dry matter (DM)), calcium oxide (CaO, 160 g/kg DM), hydrobromic acid (HBr, 60 mL/kg DM), and hydrogen peroxide (H2O2, 57 mL/kg DM) processing on the nutritional value of common bean (Phaseolus vulgaris) residues (CBR). Methods The chemical composition of the samples was determined using standard methods. In addition, a degradability trial was conducted using the nylon-bag technique. Furthermore, in vitro digestibility of the samples was determined by the batch-culture method. Moreover, the X-ray powder diffraction technique was used to calculate the crystallinity degree of the samples. Key results Chemical processing was effective on the chemical composition of the residues (P < 0.05). Treatments of CaO, H2O2, and NaOH increased the ash content. Chemicals reduced the neutral detergent fibre and acid detergent fibre. Other treatments, excluding CaO, increased total digestible nutrients, net energy for lactation, and net energy for gain. NaOH, H2O2 and HBr treatments increased the effective ruminal degradability of DM at ruminal outflow rates of 0.02, 0.05, and 0.08/h (P < 0.0001). Chemical processing caused a decrease in gas production (GP) and estimated parameters of CBR (P < 0.0001). The decrease in GP, short-chain fatty acids, metabolisable energy, and organic-matter digestibility in the NaOH and CaO treatments was more than in HBr and H2O2 treatments. After 24 h of incubation, all treatments except CaO increased the partitioning factor and decreased the gas yield (P < 0.0001). Furthermore, processing with NaOH and H2O2 increased the microbial biomass (P = 0.0128) and its efficiency (P < 0.0001) at the end of a 24 h incubation period. Moreover, different treatments reduced the crystallinity degree of CBR compared with the control. Notably, the lowest crystallinity percentage was observed in HBr-treated samples. Conclusions This study shows that chemical treatments (except for CaO) improve CBR nutritional value under in vitro and in situ conditions. Implications In vivo tests are required to validate the results.

In vitro colonic fermentation of Mexican “taco” from corn-tortilla and black beans in a Simulator of Human Microbial Ecosystem (SHIME®) system

Relevance. The study of collection samples of the common bean (Phaseolus vulgaris L.) in order to identify among them the most adapted to local conditions and designate economically valuable traits in the foothill zone of the North-Western Caucasus for the cultivation of this crop on an industrial scale.Methods. The material for this work was 106 accessions of the common bean (Phaseolus vulgaris L.) from the collection of the All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov of diverse ecological and geographical origin, that arrived in 2019–2021 and were reproduced at the Maikop Experimental Station, a branch of VIR. Research methods: study and evaluation of morphological features, phenological and statistical studies.Results. During growth and development, competitive relationships appear between plants, which directly affects their resistance to environmental conditions and the productivity of individual plants. Based on this, a detailed study of the best European and domestic varieties and the designation of economically valuable traits in the conditions of the foothill zone of the Northwestern Caucasus are very timely. The growth and development of bean plants is affected by a combination of the amount of heat and moisture during the growing season, as well as the individual response of varieties to these indicators, in this case, in the conditions of the foothill zone of the Northwestern Caucasus, due to the genotype. The main characteristics that distinguish the common bean (Phaseolus vulgaris) among food leguminous crops are its wide range in the food industry and nutritional value. The seeds of this crop contain up to 30% protein, up to 3% fat, and the protein contains all amino acids indispensable for humans nutritionally.The article presents the results of studying the collection samples of the common bean (Phaseolus vulgaris L.), identifying among them the most adapted to local conditions and designating the prospects for cultivating the crop on an industrial scale in the soil and climatic conditions of the foothill zone of the Northwestern Caucasus.