Abstract
Haemophilus parasuis (H. parasuis) can cause Glässer’s disease in pigs. However, the molecular mechanism of the inflammation response induced by H. parasuis remains unclear. The high-mobility group box 1 (HMGB1) protein is related to the pathogenesis of various infectious pathogens, but little is known about whether H. parasuis can induce the release of HMGB1 in piglet peripheral blood monocytes. Baicalin displays important anti-inflammatory and anti-microbial activities. In the present study, we investigated whether H. parasuis can trigger the secretion of HMGB1 in piglet peripheral blood monocytes and the anti-inflammatory effect of baicalin on the production of HMGB1 in peripheral blood monocytes induced by H. parasuis during the inflammation response. In addition, host cell responses stimulated by H. parasuis were determined with RNA-Seq. The RNA-Seq results showed that H. parasuis infection provokes the expression of cytokines and the activation of numerous pathways. In addition, baicalin significantly reduced the release of HMGB1 in peripheral blood monocytes induced by H. parasuis. Taken together, our study showed that H. parasuis can induce the release of HMGB1 and baicalin can inhibit HMGB1 secretion in an H. parasuis-induced peripheral blood monocytes model, which may provide a new strategy for preventing the inflammatory disorders induced by H. parasuis.
Highlights
Haemophilus parasuis (H. parasuis), the causative agent of Glässer’s disease in pigs, is a Gram-negative bacterium that colonizes the upper respiratory tract of pigs [1]
To explore the pattern of production of high-mobility group box 1 (HMGB1) promoted by H. parasuis and LPS in detail, the piglet peripheral blood monocytes were infected with H. parasuis or LPS for 12 h to 48 h
The results showed that H. parasuis could stimulate the production of HMGB1 in the piglet peripheral blood monocytes for 12 h to 48 h compared with the control cells, and the amount of HMGB1 released reached a peak at 24 h before falling at 36 h to 48 h (Figure 1A) (p < 0.05)
Summary
Haemophilus parasuis (H. parasuis), the causative agent of Glässer’s disease in pigs, is a Gram-negative bacterium that colonizes the upper respiratory tract of pigs [1]. H. parasuis can cause high morbidity and mortality, resulting in huge economic losses for the pig industry [3]. In recent years, it has become one of the most important bacterial respiratory pathogens, and has received increasing attention from pig producers. H. parasuis serovars are considered to be important markers of bacterial virulence [7,8]. Because of the large number of H. parasuis serovars and uncertainty in the identification of some of them, preventing and controlling infection by H. parasuis has become a challenge
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
