Baicalin have some effects of prevention and cure on rats with chronic obstructive pulmonary disease by regulate INF-γ

Abstract

Objective To study prevention and treatment effects of Baicalin in model rats lung with chronic obstructive pulmonary disease (COPD) by made COPD model rats.Methods According to the principle of random,25 Wistar rats (male) were divided into 5 groups,free group (group F),model contrast group (group C),maximum dose Baicalin,middling dose and minimum dose treatment groups (group T1,group T2,group T3 ). Model group was established by intratracheal instillation of lipopolysaccharide (LPS) twice and exposed to cigarette smoke. At the process of made model,gave Baicalin to treat. After treatment,killed the rats to research each group rats lung tissue HE dyeing pathological change and mean alveolar number (MAN),the ratio of dried lung weight to fresh lung weight (RDW),immunohistochemical stains were performed with INF γ to detect the expression.Results The COPD rats model were successfully made. The RDW of group F were 15.36 ± 0.43,group C were 10.86±0.51,group T1 were 13.86±0.44,group T2 were 13.16±0.42,group T3 were 12.24±0.36.The MAN of Group F were 99.00±5.90,group C were 40.00±5.90,group Tl were 65.00±5.20,group T2were 62.00 ± 6.30,group T3 were 58.00 ±4.50,INF-γ immunohistochemical stains were performed show group F were 108.71±7.85,group C were 60.94±2.21,group T1 were 98.15±3.95,group T2 were 91.17±2.45,group T3 were 69.48±2.45.Conclusions Baicalin have some effects on COPD model rats.Maybe one of the theory is regulate INF-γexpression in the lung. Key words: Baicalin; Chronic obstructive pulmonary disease; Interferon-γ; Chronic obstructive pulmonary disease animal model