Abstract
Baicalein is a type of flavonoid originated from Scutellaria baicalensis. It has been reported to have inhibitory effects in inflammation, proliferation and adipogenesis. In this study, we examined how baicalein inhibits lipid accumulation in adipogenesis of 3T3‐L1 cell. Our data showed that baicalein inhibits lipid accumulation in adipogenesis in a dose dependent manner. The inhibition of baicalein was limited to early adipogenic stage (day0–2). Trypan blue assay showed that baicalein inhibits adipocyte proliferation during day0–2, when mitotic clonal expansion (MCE) process occurs. Baicalein‐induced inhibition on MCE was shown to be derived through cell cycle arrest in G0/G1 phase of 3T3‐L1. In addition, baicalein suppressed mRNA expressions of CCAAT/ enhancer binding protein‐β (C/EBPβ), CCAAT/ enhancer binding protein‐δ (C/EBPδ), early growth response 2 (Egr2 or Krox20), Krueppel‐like factor 4 (KLF4) and Krueppel‐like factor 5 (KLF5). This result resulted in the downregulation of late adipogenic factors such as peroxisome proliferator‐activated receptor‐γ (PPARγ), CCAAT/ enhancer binding protein‐α (C/EBPα) and adipocyte protein 2 (aP2). Our data suggest that bacalein inhibit lipid accumulation by regulating MCE through cell cycle arrest as well as early adipogenic genes.Grant Funding Source: Korea and National Research Foundation of Korea
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