Abstract
Purpose: To investigate baicalein and 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene (U0126)effects on human bladder cell line T24 proliferation and related mechanisms.Methods: Twenty micromoles of baicalein or 10 μM U0126 were incubated with T24 cells. Cell viability was tested by CCK8 assay. Cell cycle was evaluated by flow cytometry while cell apoptosis was detected by Annexin V/PI and TUNEL assay. MAPK signaling pathway was evaluated by real time polymerase chain reaction (RT-PCR) and western blot.Results: Baicalein and U0126 suppressed bladder cancer cell T24 proliferation by blocking cell cycle in G0~G1 phase. TUNEL and Annexin V/PI detection showed both baicalein and U0126 induced T24 cell apoptosis. Baicalein and U0126 significantly down-regulated MAPK signaling pathway related molecule activity in both mRNA and protein levels (p < 0.05).Conclusion: Baicalein and U0126 restrain bladder cancer cell proliferation and promote cell apoptosis by affecting MAPK signaling pathway. Thus, they have potentials for use in the treatment of bladder cancer.Keywords: Bladder cancer, Baicalein, 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene, MAPK signal pathway, Apoptosis
Highlights
Bladder cancer is a common malignant cancer in the urinary system
CCK8 results demonstrated that T24 cell viability was significantly reduced by baicalein or U0126 with dose dependence (p < 0.05) (Figure 1A)
Since Mitogen-activated protein kinase (MAPK) signaling pathway can regulate cell cycle, we aimed to clarify if baicalein and U0126 impact on T24 cell cycle
Summary
Bladder cancer is a common malignant cancer in the urinary system. Bladder epithelial tumor accounts for 95~98 %, of which transitional cell carcinoma accounts for 90 %, and non-epithelial tumor including phosphorus epithelial carcinoma and adenocarcinoma occupies 2 – 5 % [1,2]. Bladder cancer occurrence and development are multi-factorial, polygenic, and multi-step processes, which is closely related to cell proliferation and apoptosis. A variety of growth factors, oncoproteins, and their interactions play important roles in cancer proliferation and apoptosis. Their imbalance is the basis of bladder cancer oncogenesis [3,4]. Inducing cell apoptosis is an important strategy for bladder cancer treatment [5]
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